Study on tissue culture of pasqueflower.
- Author:
Zi-xue ZHANG
1
;
Wei-qun DING
;
Yong TANG
;
Wei-jing SHI
;
Wen-cai YE
Author Information
- Publication Type:Journal Article
- MeSH: Flowers; growth & development; Plant Growth Regulators; pharmacology; Plant Roots; growth & development; Plant Stems; growth & development; Plants, Medicinal; growth & development; Pulsatilla; growth & development; Seedlings; growth & development; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2004;29(3):215-218
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo speed up seedling production of pasqueflower (Puzlsatilla chinenses) and their modernization in pasqueflower.
METHODWith tissue culture method, primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment combinations of adding different hormones.
RESULTThe appropriate medium for different culture stages were MS + 6-BA 1.0-3.0 mg x L(-1) + NAA 0-0.05 mg x L(-1) + Sucrose 30 g x L(-1) in primary culture, MS + 6-BA 0.2 mg x L(-1) + NAA 0.02 mg x L(-1) + BR 0.00001 mg x L(-1) + Sucrose 30 g x L(-1) in differentiation and subculture of cluster buds, 1/2 MS + NAA 0.4 mg x L(-1) + Sucrose 20 g x L(-1) in rooting.
CONCLUSIONApplying stem tip and flower buds as explants, high frequency propagation of seedlings can be achieved with plant tissue culture in Pasqueflower.
