Study on optimizing extraction process of root of Polygonum multiflorum.
- Author:
Hai-bo BAI
1
;
Jian-fei WANG
;
Juan LONG
Author Information
- Publication Type:Journal Article
- MeSH: Antioxidants; isolation & purification; pharmacology; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; isolation & purification; pharmacology; Ethanol; Glucosides; analysis; isolation & purification; Plant Roots; chemistry; Plants, Medicinal; chemistry; Polygonum; chemistry; Stilbenes; analysis; isolation & purification; Technology, Pharmaceutical; methods; Time Factors
- From: China Journal of Chinese Materia Medica 2004;29(3):219-221
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the extraction process of root of Polygoni multflori and validate the interrelation between antioxdative capacity and the content of 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside in root of P. multiflori.
METHODThe optimum extraction was abserved with the orthogonal design; 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside was determined by HPLC and the antioxidative capacity by using photochemiluminescence detection method with the Photochem supplied by Analytik Jena AG; the concentration of ethanol, amount of ethanol, extraction time and extraction times were the four factors in the extraction.
RESULTThe concentration of ethanol and extraction times had significant effect on the content of 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside and the antioxidative capacity of the crude extract.
CONCLUSIONThe best extraction process is to extract three times by using 10 fold EtOH(60%), refluxing at 85 degrees C and to extract one and half hours each time.
