Extraction of coumarins from Angelica dahurica by supercritical extraction and GC-MS analysis.
- Author:
Hong-mei LIU
1
;
Ming-xian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Angelica; chemistry; Carbon Dioxide; Chromatography, Supercritical Fluid; Coumarins; chemistry; isolation & purification; Furocoumarins; analysis; Gas Chromatography-Mass Spectrometry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo extract coumarins from Angelica Dahurica by supercritical CO2.
METHODSix-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. Constituents of the coumarins were analyzed by GC-MS, and their relative contents were determined by normalization method of areas.
RESULTTotal 15 coumarins were separated, the relative contents of oxyimperatorin, imperatorin and isoimperatorin being up to 42.40%, 22.14% and 12.12% respectively. Optimum extraction process was established: 21 MPa as extraction pressure, 50 degrees C as extraction temperature, 3 h as time for extraction, 20 mesh as pulverized degree, 6.5 MPa as separation pressure and 30 degrees C as separation temperature.
CONCLUSIONChanges in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably.
