Influence of sargassum fusiforme polysaccharide on apoptosis of tumor cells.

Author: Yu-bin JI ¹ ; Shi-yong GAO ; Xiu-juan ZHANG
Author Information

1. Postdoctoral Programme, the Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China. jyb@hrbcu.edu.cn
Publication Type:Journal Article
MeSH: Antineoplastic Agents; isolation & purification; pharmacology; Apoptosis; drug effects; Calcium; metabolism; Calcium Chloride; pharmacology; Cell Cycle; drug effects; Humans; Polysaccharides; isolation & purification; pharmacology; Sargassum; chemistry; Stomach Neoplasms; metabolism; pathology; Tumor Cells, Cultured
From: China Journal of Chinese Materia Medica 2004;29(3):245-247
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study mechanism of antitumor activity of Sargassum Fusiforme Polysaccharide (SFPS).
METHODThe effect on cell cycle and apoptosis was studied with flow cytometry (FCM). Intracellular calcium concentration [Ca2+]i was marked with Fluo-3/AM and measured with laser scanner confocal microscope (LSCM).
RESULTSFPS inhibited G0/G1 stage SGC-7901 from entering to S stage and increased APO%. The [Ca2+]i showed a transient rise and return to the original level. The concentration could be raised again by administering CaCl2.
CONCLUSIONThe antitumor effect of SFPS seems to be accomplished through the apoptosis associated with the increase in intracellular calcium concentration. Intracellular stores release the calcium during its action.