Effect of puerarin on L-type calcium channel in isolated rat ventricular myocytes.
- Author:
Xiao-gang GUO
1
;
Jun-zhu CHEN
;
Xiong ZHANG
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Arrhythmia Agents; isolation & purification; pharmacology; Calcium Channels, L-Type; drug effects; Cell Separation; Heart Ventricles; cytology; Isoflavones; isolation & purification; pharmacology; Male; Myocytes, Cardiac; metabolism; Patch-Clamp Techniques; Plants, Medicinal; chemistry; Pueraria; chemistry; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2004;29(3):248-251
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Puerarin on L-type calcium channel in isolated rat ventricular myocytes.
METHODThe cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion techniques at constant flow rate. Whole-cell recording of patch-clamp techniques was used to observe the current of L-type calcium channel.
RESULTPuerarin 2.4 mmol x L(-1) could inhibit the current of L-type calcium channel of rat ventricular myocytes and this inhibition was time-dependent. Purerarin elevated the current-voltage (I-V) curve of calcium current.
CONCLUSIONPuerarin can inhibit L-type calcium current of rat ventricular myocytes. Which implies that puerarin takes part in anti-myocardial ischemia and anti-arrhythmics partly due to the inhibition of L-type calcium channel.
