The effect of lishi No.5 formula on the growth of human neuroblastoma cell line SY5Y.
- Author:
Jing-yan ZHANG
1
;
Rong WANG
;
Zi-zhong LI
;
Hao-ran CHENG
;
Sheng-hai LI
;
Meng-xia LIU
;
Shu-li SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; drug effects; Cell Survival; drug effects; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Humans; L-Lactate Dehydrogenase; metabolism; Neuroblastoma; metabolism; pathology; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(3):258-260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Lishi No.5 formula on the growth of human neuroblastoma cell line SY5Y and find out the most effective drug dose.
METHODSY5Y cells were administrated by Lishi No.5 formula in different concentration. MTT metabolic rate was measured as the cell survival rate, then the dose-effect curve was made. LDH leakage rate, axonal length and area of cell body were used as the indicators.
RESULTIn 0.125-0.75 g x L(-1) Lishi No. 5 formula could increase the cell survival rate and MTT metabolic rate, decreased LDH leakage rate, and enhance axonal length and area of cell body, compared with control group.
CONCLUSIONLishi No.5 formula has the neurotrophic effect and can induce the survival of neuron.
