Studies on influence of fungal elicitor on hairy root of Panax ginseng biosynthesis ginseng saponin and biomass.
- Author:
Jun LIU
1
;
Jia-yi DING
;
Qian-yun ZHOU
;
Lin HE
;
Zheng-tao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Aspergillus niger; chemistry; physiology; Coculture Techniques; Fusarium; chemistry; physiology; Ginsenosides; biosynthesis; Panax; growth & development; metabolism; microbiology; Plant Roots; growth & development; metabolism; microbiology; Plants, Medicinal; growth & development; metabolism; microbiology; Polysaccharides; isolation & purification; pharmacology
- From: China Journal of Chinese Materia Medica 2004;29(4):302-305
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study influence of fungal elicitors on the biomass and ginseng saponin biosynthesis of hairy roots of Panax ginseng (HRPG).
METHODFungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV-spectrophotometry and RP-HPLC.
RESULTFungal elicitors coula not only can influence on HRPG biomass and total ginseng sponin, but also improve or decrease some monomeric sponin. The total ginseng sponin could be increased to 3.649% but Rg1 and Re could not be detected when A. niger elicitors wss 20 mg x L(-1) in the culture fluid.
CONCLUSIONFungal elicitor has specificity influence on secondary metabolite of HRPG. HRPG can biosynthesize specially active component by using specific fungal elicitor is used.
