Screening of angiotensin converting enzyme inhibitors from Salvia miltiorrhizae.
- Author:
Xiao-ping GAO
1
;
Da-yong XU
;
Yi-long DENG
;
Yan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin-Converting Enzyme Inhibitors; isolation & purification; pharmacology; Animals; Benzofurans; isolation & purification; pharmacology; Drugs, Chinese Herbal; isolation & purification; pharmacology; In Vitro Techniques; Lung; metabolism; Peptidyl-Dipeptidase A; metabolism; Plant Roots; chemistry; Plants, Medicinal; chemistry; Rabbits; Rats; Salvia miltiorrhiza; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(4):359-362
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen angiotensin converting enzyme inhibitor (ACEI) from traditional Chinese medicine, Salvia miltiorrhiza.
METHODHydrophilic and lipophilic fractions of S. miltiorrhiza were isolated, and their effective components were screened by a fluorimetric assay for inhibition of angiotensin converting enzyme (ACE).
RESULTWater-soluble fractions, total salvianolic acids and salvianolic acid B markedly decreased ACE activity of rabbit lung tissue. Their IC50 value were (2.45 +/- 0.07), (0.24 +/- 0.02), (0.02 +/- 0.01) g x L(-1) respectively. Lipophilic components or phenanthraquinones including tanshinone I and II showed no changes on the activity of ACE.
CONCLUSIONS. miltiorrhiza inhibits angiotensin converting enzyme and its active components are in aqueous extract, in which the main were salvianolic acids including salvianolic acids B.
