Determination of aristolochic acid A in Radix Aristolociae and Herba Asari by RP-HPLC.
- Author:
Xu JIANG
1
;
Zhi-min WANG
;
Li-shuan YOU
;
Li-ping DAI
;
Guang-zhi DING
Author Information
- Publication Type:Journal Article
- MeSH: Aristolochia; chemistry; Aristolochic Acids; analysis; Asarum; chemistry; China; Chromatography, High Pressure Liquid; methods; Ecosystem; Plant Roots; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(5):408-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method to determine the contents of aristolochic A in aristolochia debilis and Asarun spp..
METHODMethanol-water-formic acid extracts were separated on an Alltech C18 column with methanol-water-acetic acid (68:32:1) as mobile phase. The flow rate was 1.0 mL x min(-1). UV detection wavelength was 390 nm. Column temperature was 35 degrees C.
RESULTAristolochic acid A was separated well. The relationship of injection amounts and peak areas was linear (r = 0.9999) the range of 0.12-1.89 microg x g(-1) and the recovery rate was 101.8% (n = 5). 11 samples of aristolochia debilis which bought from different areas in China were determined, and the contents of aristolochic acid A varied from 0.9 to 2 mg x g(-1). The difference of the contents in Asarum spp. was obvious. The highest is 0.35, and aristolochic acid A couldn't be detected in one sample.
