Study on extraction technique of HDCA.
- Author:
Jin LU
1
;
Shou-ying DU
;
Li-rui ZHAO
;
Wei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bile; chemistry; Chromatography, Thin Layer; Deoxycholic Acid; analysis; isolation & purification; Materia Medica; analysis; isolation & purification; Swine; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2004;29(5):414-417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo find out the optimal extraction technique for HDCA (a-hydroxycholic acid).
METHODAccording to the orthogonal design L9(3(4)), the optimal extraction technique was sought through experimental investigation, and the content of HDCA was determined by TLC.
RESULTThe optimal extraction method was eightfold 8% NaOH solution and 16 hours. The optimal purification method was six fold ethyl acetate, 5% active carbon, and 30 minutes twice.
CONCLUSIONThe above mentioned extraction technique is optimal and feasible in extraction of HDCA.
