Experimental study on antivirus activity of traditional Chinese medicine.
- Author:
Chao-man HE
1
;
Liang-zhen WEN
Author Information
- Publication Type:Journal Article
- MeSH: Antiviral Agents; pharmacology; Cells, Cultured; Cytomegalovirus; drug effects; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Fibroblasts; virology; Humans; Inhibitory Concentration 50; Isatis; chemistry; Lonicera; chemistry; Lung; cytology; Plant Leaves; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Saururaceae; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(5):452-455
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the difference of anticytomegaloviral activity of three kinds of traditional Chinese medicines which are the injections of Jinye Baidu, Radix Isatidis and Indigowoa in vitro.
METHODThe inhibitory activity of three traditional Chinese medicines against human cytomeglovirus (HCMV AD169) infected human embryo lung fibroblasts (HELF) was observed by cytopathic effect method (CPE) and MTT method in vitro. According their value of A, anticytomegaloviral activity has evaluated.
RESULTExperimental study in vitro showed that the 50% toxicity dose (TD50) of Jinye Detoxifying, Radix Isatidis root and Indigowoa were 20, 10.23, 20.23 g x L(-1) respectively; the 50% inhibitory concertration (IC50) were 5.65, 3, 5.71 g x L(-1) respectively; the therapeutic index (TI) were 3.54, 3.41 and 3.54 respectively. It suggested that three traditional Chinese medicines had anticytomeglovirus activity and their effect increased with their concentration.
CONCLUSIONThree traditional Chinese medicines of the parenteral solution of Jinye Detoxifying, Radix Isatidis root and Indigowoa have antiviral activity when they are diluted in 1:200. They are safe and valuable drug for inhibiting cytomeglovirus infection.
