Determination of flavoniod-glycosides in Yixintong pills by HPLC.
- Author:
Juan SU
1
;
Wei-dong ZHANG
;
Yun ZHOU
;
Jing ZHOU
;
Zheng-bing GU
Author Information
- Publication Type:Journal Article
- MeSH: Apigenin; analysis; Chromatography, High Pressure Liquid; Crataegus; chemistry; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Plant Leaves; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2004;29(6):525-527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determining flavoniod-glycosides in Yixintong pills.
METHODAgilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30:3:4:120:3) as mobile phase. Detection wavelength was 330 nm.
RESULTGood linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively.
CONCLUSIONThis method can be used for quality control of Yixintong pills.
