Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC.
- Author:
Xian-duan LI
1
;
Zhi-jing MA
;
Sheng LIN
;
Xue-zhu GU
;
Shu-jie MAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Citrus; chemistry; Fruit; chemistry; Plants, Medicinal; chemistry; Quality Control; Seasons; Synephrine; analysis; Tyramine; analogs & derivatives; analysis
- From: China Journal of Chinese Materia Medica 2004;29(6):537-539
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.
METHODSamples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.1) as mobile phase. Detection wavelength was 285 nm.
RESULTSynephrine and N-methyltyramine in sample solution were well separated. Linearity of synephrine was good (r = 0.9999) in range of 0.35-11.24 microg. The average recovery was 97.1%, and RSD of repeatability was 1.9%.
CONCLUSIONThis method can be used for quality control of Citri Reticulatae.
