DNA microarray analysis of protective mechanism of buyang huanwu decoction on focal cerebral ischemia/reperfusion in rats.
- Author:
Tie-jun LI
1
;
Yaen QIU
;
Yao-cheng RUI
;
Ting-zhao LI
;
Wei-dong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Ischemia; complications; genetics; Drugs, Chinese Herbal; pharmacology; Gene Expression Profiling; Male; Neuroprotective Agents; pharmacology; Oligonucleotide Array Sequence Analysis; Rats; Rats, Sprague-Dawley; Reperfusion Injury; etiology; genetics
- From: China Journal of Chinese Materia Medica 2004;29(6):559-563
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyse the effects of buyang huanwu decoction (BYHWT) on differentially expressed genes during cerebral ischemia/reperfusion in rats with DNA microarray.
METHODcDNA microarray chips containing 512 cDNAs were made by Biostar Genechip Inc. Sprague-Dawley rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) with an filament. Saline or BYHWT was given p.o. after onset of cerebral ischemia and brains were removed after 24 h of recirculation for mRNAs isolation. A differential measurment of mRNAs from post-ischemic and BYHWT treated animals was performed with microarray.
RESULTUp-and down-regulated genes were 69 and 80 in ischemic group. Up-and down-regulated genes were 25 and 6 in BYHWT treated group.
CONCLUSIONBYHWT regulates the differential expression genes after focal brain ischemia/reperfusion in rats, due to its mechanism of protecting cerebral ischemia/reperfusion injury.
