Effects of ezrin silencing on pancreatic cancer cell line Panc-1.
- Author:
Yun-xiao MENG
1
;
Shuang-ni YU
;
Zhao-hui LU
;
Jie CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Movement; Cell Proliferation; Cell Surface Extensions; pathology; Cytoskeletal Proteins; genetics; metabolism; Humans; Microvilli; pathology; Neoplasm Invasiveness; Pancreatic Neoplasms; genetics; metabolism; pathology; Plasmids; RNA Interference; RNA, Small Interfering; Transfection
- From: Chinese Journal of Pathology 2012;41(12):833-836
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.
METHODSPancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid. The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis, respectively. Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy. Colony formation assay was used to determine the cell anchor-independent growth ability in vitro. Trans-filter migration and invasion assays were performed with 8 µm pore inserts in a 24-well BioCoat chamber with/without Matrigel.
RESULTSEzrin silencing decreased cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion, but had no effects on cell proliferation in vitro and cell cycle, in pancreatic cancer cell line Panc-1.
CONCLUSIONEzrin expression affects the cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion in pancreatic cancer cell line Panc-1.
