- VernacularTitle:胃癌中microRNA-100的表达
- Author:
Duan-bo SHI
1
;
Ai-yan XING
;
Chao GAO
;
Peng GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cell Line, Tumor; Cell Movement; Cell Proliferation; Female; Humans; Lymphatic Metastasis; Male; MicroRNAs; genetics; metabolism; Middle Aged; Neoplasm Invasiveness; Stomach Neoplasms; genetics; pathology; Transfection
- From: Chinese Journal of Pathology 2013;42(1):15-19
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of microRNA-100 (miR-100) in human gastric cancer cells and its role of miR-100 in migration, invasion and proliferation in gastric cancer cell line SGC7901.
METHODSTotal RNAs were extracted from formalin-fixed and paraffin-embedded tissue samples of SGC7901 cells, gastric cancer (50 cases), non-tumor (18 cases) and lymph nodes with metastases (18 cases). The expression of miR-100 was examined by reverse transcription (RT)-qPCR. Additionally, SGC7901 cells were transfected with Pre-miR-100 and negative control constructs, and then their ability of migration, invasion and proliferation in vitro was documented after 48 hours.
RESULTSRT-qPCR showed that although miR-100 expressed in all samples, compared to non-tumour tissues, the expression was lower both in SGC7901 cells and gastric cancer tissues (P = 0.0077, P < 0.01). SGC7901 cells and primary gastric cancer tissues with lymph nodes metastasis had lower miR-100 expression than those of without lymph node metastasis (P = 0.0361, P = 0.0356). The migration ability and invasion of SGC7901 cells transfeced with pre-miR-100 decreased as compared with control cells (P = 0.0025, P = 0.0028 respectively). However, miR-100 expression had no significant effects on the cell proliferation.
CONCLUSIONSExpression of miR-100 inhibits the migration and invasion of gastric cancer cells without significant alteration of proliferation. Therefore, miR-100 may play an inhibitory role in the progression of gastric carcinoma.