Immunohistochemistry using epidermal growth factor receptor mutation-specific antibodies of delE746-A750 and L858R in lung adenocarcinomas.
- Author:
Xiang-shan FAN
1
;
Biao LIU
;
Bo YU
;
Shan-shan SHI
;
Xuan WANG
;
Jin ZHANG
;
Jian-dong WANG
;
Zhen-feng LU
;
Heng-hui MA
;
Xiao-jun ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; metabolism; pathology; Adult; Aged; Aged, 80 and over; Antibodies, Monoclonal; DNA Mutational Analysis; Female; Gene Deletion; Humans; Immunohistochemistry; Lung Neoplasms; genetics; metabolism; pathology; Male; Middle Aged; Paraffin Embedding; Point Mutation; Receptor, Epidermal Growth Factor; genetics; immunology; metabolism; Sensitivity and Specificity
- From: Chinese Journal of Pathology 2013;42(3):173-177
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the immunohistochemical detection of epidermal growth factor receptor(EGFR) mutations using two EGFR mutation-specific monoclonal antibodies: delE746-A750 and L858R.
METHODSA total of 175 paraffin-embedded lung adenocarcinoma tissue samples previously genotyped by directive DNA sequencing were subject to immunostaining using delE746-A750 and L858R antibodies.
RESULTSThere was no significant difference of mutation detection between DNA sequence analysis and delE746-A750 and/or L858R immunostaining (33.7% vs 30.9%, P > 0.05). The overall sensitivity, specificity, positive predictive value and negative predictive value of immunostaining using these two EGFR mutation-specific antibodies were 83.1%, 95.7%, 90.7% and 90.9%, respectively.
CONCLUSIONWith high sensitivity and good specificity, immunohistochemistry using EGFR mutation-specific monoclonal antibodies is an adequate, easy and cost-effective prescreening method to detect EGFR mutations using paraffin-embedded tissue specimens of lung adenocarcinomas.
