Optimization of cultivation conditions in se-enriched Spirulina platensis.
- Author:
Zhi HUANG
1
;
Wen-Jie ZHENG
;
Bao-Jiang GUO
Author Information
1. Department of Biotechnology, Department of Chemistry, Jinan University, Guangzhou 510632.
- Publication Type:Journal Article
- MeSH:
Bicarbonates;
analysis;
Culture Media;
Cyanobacteria;
growth & development;
Selenium;
analysis;
pharmacology
- From:
Chinese Journal of Biotechnology
2002;18(3):373-376
- CountryChina
- Language:Chinese
-
Abstract:
Orthogonal combination design was adopted in examining the Spirulina platensis (S. platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO3 content) on algae growth. The results showed that Se content, Se-adding method and NaHCO3 content were key factors in cultivation conditions of Se-enriched S. platensis with the optimal combination being Se at 300 mg/L, Se-adding amount equally divided into three times and NaHCO3 at 16.8 g/L. Algae yield had a remarkable correlation with OD560 and floating rate by linear regression analysis. There was a corresponding relationship between effects of the four factors on algae yield and on OD560, floating rate too. In conclusion, OD560 and floating rate could be served as yield-forming factors.