Agroinfiltration, a useful technique in plant molecular biology research.
- Author:
Zhao-Ming LIU
1
;
Zong-Zhi LIU
;
Qing-Wu BAI
;
Rong-Xiang FANG
Author Information
1. Institute of Microbiology, Chinese Academy of Science, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Agrobacterium tumefaciens;
genetics;
Genetic Vectors;
genetics;
Plants;
genetics;
Plants, Genetically Modified;
Research Design
- From:
Chinese Journal of Biotechnology
2002;18(4):411-414
- CountryChina
- Language:Chinese
-
Abstract:
Agroinfiltration is a newly developed plant transient gene expression technique, which is simple, rapid and reproducible. It has been widely used in analyses of foreign gene expression, hypersensitive reaction, gene silencing, promoter activity and identification of new disease-resistance genes. In this paper, we describe the principle and the operation procedure of Agroinfiltration and its application in diverse aspects of plant molecular biology research. Our experiences in modification of the Agroinfiltration technique are also provided.
