Construction of a temperature inducible shuttle expression vector and its application in Streptomyces.
- Author:
Mei-Feng TAO
1
;
Xiu-Fen ZHOU
;
Tobias KIESER
;
Zi-Xin DENG
Author Information
1. Key Laboratory of Agricultural Microbiology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Blotting, Western;
Cloning, Molecular;
Genetic Vectors;
genetics;
Molecular Sequence Data;
Multienzyme Complexes;
genetics;
metabolism;
Recombinant Proteins;
genetics;
metabolism;
Streptomyces;
enzymology;
genetics;
Temperature
- From:
Chinese Journal of Biotechnology
2002;18(4):420-423
- CountryChina
- Language:Chinese
-
Abstract:
pHZ1080, an E. coli-Streptomyces shuttle expression vector was constructed in order to explore the utilization of lambda phage regulated expression elements in Streptomyces. A 2.7 kb polyketide synthase (PKS) gene from Streptomyces sp. FR-008 was inserted into downstream of lambda phage promoter (PR) to give the shuttle plasmid, pHZ1067. The PKS protein was expressed in Streptomyces lividans carrying pHZ1067 in a heat-dependent manner, as it did in E. coli. The PKS protein expressed in both hosts with same molecular weight was detected by SDS-PAGE and Western-blot. The successful heat-induced expression of PKS suggested that pHZ1080 was useful and convenient for heat-induced expression of heterologous genes in both E. coli and Streptomyces.
