Detection of P53 and K-ras gene mutations in lung cancer with oligonucleotide chip.
- Author:
Jun-Bo LIU
1
;
Ji-Pan XIE
;
Zong-Liang ZOU
;
Lin-Jie CHEN
;
Long-Yun LI
;
Sheng-Qi WANG
Author Information
1. ShenZhen Yishengtang Biopharmceutical Co., Ltd., Shenzhen 518260, China.
- Publication Type:Journal Article
- MeSH:
Genes, ras;
genetics;
Humans;
Lung Neoplasms;
genetics;
pathology;
Mutation;
Oligonucleotide Array Sequence Analysis;
methods;
Oligonucleotides;
genetics;
Sensitivity and Specificity;
Tumor Suppressor Protein p53;
genetics
- From:
Chinese Journal of Biotechnology
2002;18(4):447-451
- CountryChina
- Language:Chinese
-
Abstract:
Different factors including hybridization solution components, hybridization temperature, and the concentration and proportion of the labelled primer, which affected the sensitivity and specificity of single mutation identification, were exploited. Asymmetric PCR increased the hybridization sensitivity, and the asymmetric multi-PCR did not affect the specificity, while the sensitivity was improved a little. Among 30 lung cancer samples detected with the oligonucleotide microarray, 12 was found P53 gene mutations and 5 had K-ras gene mutations. The P53 gene mutations identified by the oligonucleotide microarray was proved 80% same as the sequencing results. The obvious statistical relations of K-ras and P53 gene mutations with tumor type, tumor stage and smoking were not obtained because of less samples and mutation sites.
