Isolation, expression analysis of a chilling induced cDNA from rice root with differential display: an evidence role for caffeine-sensitive calcium signal.
- Author:
Kui-De YIN
1
;
Xing-Mei ZHANG
;
Shi-Qiang LIU
;
Le-Gong LI
Author Information
1. Heilongjiang August First Reclamation Agriculture University, Mishan 158308, China. Yinkuide@sohu.com
- Publication Type:Journal Article
- MeSH:
Cold Temperature;
DNA, Complementary;
genetics;
isolation & purification;
Gene Expression Profiling;
Gene Expression Regulation, Plant;
drug effects;
Mannitol;
pharmacology;
Oryza;
drug effects;
genetics;
Plant Roots;
drug effects;
genetics;
RNA, Messenger;
drug effects;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2002;18(4):468-471
- CountryChina
- Language:Chinese
-
Abstract:
Chilling-sensitive rice varieties acquire chilling tolerance when their roots are exposed to water stress for short time. Caffeine-sensitive calcium signal was involved in this procedure. By using total RNA differential display, a chilling induced cDNA(ICT: induction of chilling treatment) was isolated from roots of chilling-sensitive rice variety. It was determined that it is a novel cDNA by homology searching. The transcript level of ict mRNA is up-regulated under chilling stress, it is decreased to low level when the samples were transferred to standard culture conditions. Pre-treated with mannitol for two hours is beneficial to inducing ICT level of expression. This chilling induction was inhibited by caffeine, suggesting that it may play a putative role in signal transduction of caffeine-sensitive calcium.
