Expression of polyprotein of infectious bursal disease virus in Bombyx mori.
- Author:
Mi-Jia LU
1
;
Jian-Rong LI
;
Ying-Fei WANG
;
Yong-Feng JIN
;
Lian YU
Author Information
1. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blotting, Western;
Bombyx;
genetics;
virology;
Electrophoresis, Polyacrylamide Gel;
Enzyme-Linked Immunosorbent Assay;
Gene Expression;
Genetic Vectors;
genetics;
Infectious bursal disease virus;
genetics;
Polyproteins;
genetics;
metabolism;
Viral Proteins;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2002;18(4):472-476
- CountryChina
- Language:Chinese
-
Abstract:
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.
