The methods of identifying differences in mRNA expression.
- Author:
Wei HUANG
1
;
Xiao-Dong FANG
;
Wen-Ming ZHAO
;
Qi-Feng LIN
Author Information
1. Life Science and Technology Institute of Xi'an Jiaotong University, Xi'an 710049, China.
- Publication Type:Journal Article
- MeSH:
Gene Expression Profiling;
methods;
Nucleic Acid Hybridization;
methods;
Oligonucleotide Array Sequence Analysis;
methods;
RNA, Messenger;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
methods
- From:
Chinese Journal of Biotechnology
2002;18(4):521-524
- CountryChina
- Language:Chinese
-
Abstract:
Identifying changes in gene expression is of prime interest in molecular biology. A variety of methods have been developed for analyzing differentially expressed genes. Among these techniques, differential display reverse transcription PCR (DDRT-PCR), representational difference analysis (RDA) and suppression subtractive hybridization (SSH), which were based on differentially screening and subtractive hybridization technology, are applied most widely. Gene chips (gene microarrays) for analyzing gene expression patterns on larger-scale had also been developed. Here we present a review of these methods.
