Regulation of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalI) by hepatitis B virus MHBst/HBx transactivator.
- Author:
Hui-Ping DING
1
;
Jun-Qi WANG
;
Cheng JIN
Author Information
1. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Gene Expression Regulation, Enzymologic;
Hepatitis B Surface Antigens;
genetics;
physiology;
Hepatitis B virus;
Promoter Regions, Genetic;
Sialyltransferases;
genetics;
Trans-Activators;
genetics;
physiology;
Transfection
- From:
Chinese Journal of Biotechnology
2002;18(5):551-555
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis B virus MHBst and HBx fragments were amplified to construct eukaryotic expression vector pCDNA3.1-MHBst and pCDNA3.1-HBx. ST3GalI promoter region was obtained by the method of PCR and GFP report plasmid pEGFP-N1-Psial was constructed. pCDNA3.1-MHBst or pCDNA3.1-HBx with pEGFP-N1-Psial were transiently co-transfected into QGY-7701 cells using calcium phosphate-DNA co-precipitation, respectively. The expressions of Psial-directed GFP were analyzed by FAC-Scalibur. It was found that MHBst/HBx could upregulate ST3GalI promoter activity by 35.2% and 43.8%, respectively. We report the regulation of ST3GalI by MHBst and HBx transactivators. It would be helpful to further investigate the relation between hepatitis B virus infection and sialyltransferase expression.
