Screening of TNF-alpha antagonist peptides from a random peptide library displayed with Escherichia coli flagellar.
- Author:
Chao LI
1
;
Yan-Rong ZHOU
;
Du-Sheng CHENG
;
Pei-Tang HUANG
Author Information
1. Beijing Institute of Biotechnology, Beijing 100071, China. lichao@nic.bmi.ac.cn
- Publication Type:Journal Article
- MeSH:
Escherichia coli;
genetics;
Peptide Library;
Peptides;
isolation & purification;
pharmacology;
Tumor Necrosis Factor-alpha;
antagonists & inhibitors
- From:
Chinese Journal of Biotechnology
2002;18(5):556-560
- CountryChina
- Language:Chinese
-
Abstract:
Tumor necrosis factor(TNF-alpha) plays an improtant role in the process of anti-infection and anti-cancer. It can both protect and make damage to the host. In order to find new way of inhibiting its host-damaging activity, An E. coli flagella displayed random peptide library was constructed and TNF-alpha antagonist peptides were screened using the peptide library. After 5 rounds of panning and DNA sequencing, six peptide sequences were obtained. Two of them(TBP2, TBP3) have the same sequence frame of V--N-WG. After primary comparation of TNF-alpha binding ability, four peptides were synthesised and purified with RP-HPLC. The activity of inhibiting TNF-alpha was detected with L929 cell and MTT method. The data show that TBP2 and TBP3 can inhibit 90% of TNF-alpha activity when TNF-alpha gives about 30% cell toxicity on L929. The two sequences have not been reported.
