Cloning of the major antigen region of E2 gene of hog cholera virus and expression in Escherichia coli.
- Author:
Yong-Guo ZHANG
1
;
Xiang-Tao LIU
;
Xue-Qing HAN
;
Xi-Cheng LIU
;
Yan-Ming ZHANG
;
Qing-Ge XIE
Author Information
1. Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Cloning, Molecular;
Escherichia coli;
genetics;
Polymerase Chain Reaction;
Recombinant Proteins;
biosynthesis;
Viral Envelope Proteins;
biosynthesis;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2002;18(5):605-608
- CountryChina
- Language:Chinese
-
Abstract:
The major antigen region of E2 gene of Hog Cholera Prevalent Strain (Guangxi Yuling Strain) and Chinese Hog Cholera Lapinised Virus (C-strain) derived from hog and rabbit spleen tissue, was amplified by reverse transcription polymerase chain reaction(RT-PCR) and the nested Polymerase Chain Reaction (nPCR). After the amplified fragments were cloned into the expression vector pPROEX-HTb, the recombinant plasmids pPROEX-GXYL and pPROEX-C were obtained. The insert position, the size and the reading frame were right by PCR, restriction digestion and the sequence analysis. SDS-PAGE indicated that both of the reciepient germs transducted and induced by the recombinant plasmids pPROEX-GXYL and pPROEX-C could express the major antigen region of E2 gene. Western-blot indicated that the expressed antigen protein could be recognized by the positive serum of CSFV.
