Biosynthesis and accumulation of poly(3-hydroxybutyrate) in Vibrio natriegens.
- Author:
Shuang-Jiang LIU
1
Author Information
1. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. shuangjiang@hotmail.com
- Publication Type:Journal Article
- MeSH:
Cerulenin;
pharmacology;
Hydroxybutyrates;
metabolism;
Polyesters;
metabolism;
Vibrio;
metabolism
- From:
Chinese Journal of Biotechnology
2002;18(5):614-618
- CountryChina
- Language:Chinese
-
Abstract:
Accumulation of poly(3-hydroxybutyrate) [poly(3HB)] by V. natriegens was studied. Results indicated that V. natriegens used glucose, gluconate, fructose and molasses as carbon sources for poly(3HB) synthesis. When molasses was used, up to 28.4% of poly(3HB) to cellular dry weight was accumulated. The accumulation of poly(3HB) followed, was not simultaneously to, the cell growth. Analysis of the PHA polymerase, beta-ketothiolase, and acetoacetyl-CoA reductase showed that the poly(3HB) accumulation was correlated to the increase of their activities in cells. Poly(3HB) accumulation was also related to the de novo fatty acid synthesis, as revealed by the results that cerulenin, a specific inhibitor to the de novo fatty acid synthesis, significantly reduced accumulation of poly(3HB). Based on the results from this study, the synthetic pathway of poly(3HB) was proposed.
