Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes.
- Author:
Hai-Qiang YU
1
;
Yan ZHOU
;
Ping HUA
;
Wen-Song TAN
Author Information
1. State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 200237, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium;
pharmacology;
Cell Adhesion;
drug effects;
Cell Differentiation;
Cell Division;
drug effects;
Cells, Cultured;
Cellular Senescence;
drug effects;
Epidermis;
cytology;
Keratinocytes;
cytology;
drug effects;
Mice
- From:Chinese Journal of Biotechnology
2002;18(5):626-629
- CountryChina
- Language:Chinese
-
Abstract:
The effect of the calcium ion (Ca2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2 mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8%, 30.8%, 5.1%, and 26.8%, respectively. However, the Ca2+ concentrations in the medium above 0.6 mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.
