Induction of petal-like structures from petals of Crocus sativus L. and the differentiation of style-stigma-like structures in vitro.
- Author:
Li WANG
1
;
Yi LI
;
Xiang-Jun DONG
;
Wen-Hua XU
;
Bao-Chen ZHANG
Author Information
1. Northwest Plateau Institution of Biology, Chinese Academy of Sciences, Xining 810001, China. liyi@mail.nwpb.ac.cn
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Crocus;
growth & development;
Culture Media
- From:
Chinese Journal of Biotechnology
2002;18(5):638-640
- CountryChina
- Language:Chinese
-
Abstract:
Firstly the petal of Crocus sativus L was cultured on the medium that supplemented with different combinations of hormones. The petal-like structures(PLS) were induced on medium, but the induction rates were different in various medium. The highest induction rate of petal-like structures was obtained on the media that was supplemented with NAA (4 mg/L) and KT (8 mg/L). The petal-like structures were subcultured on another media when the structure was produced on the explants and proliferate groups. The later media was used for inducing style-stigma-like structures(SSLS). The induction rate of style-stigma-like-structures in the petal-like structures group is much higher than the rate in the preceding work, and the maximum of style-stigma-like structures produced per explant was 30. The best result of style-stigma-like structures was observed on the petal-like structure groups which came from the third treatment. The differentiation rate of style-stigma-like structures is stable in the subcultures of petal-like structures. The result revealed that the induction frequency of style-stigma-like structures formed on the petal-like structures is higher than that form on the petals of C. sativus L.
