Membrane-bound p35 Subunit of IL-12 on Tumor Cells is Functionally Equivalent to Membrane-bound Heterodimeric Single Chain IL-12 for Induction of Anti-tumor Immunity.
- Author:
Hyun Jin KIM
1
;
Sang Min PARK
;
Hayyoung LEE
;
Young Sang KIM
Author Information
- Publication Type:Brief Communication
- Keywords: Interleukin-12; Membrane-bound form; Cytokine gene therapy; Anti-tumor immunity
- MeSH: Clone Cells; In Vitro Techniques; Interleukin-12*; Vaccines
- From:Immune Network 2016;16(5):305-310
- CountryRepublic of Korea
- Language:English
- Abstract: In this study, we compared two different tumor cell vaccines for their induction of anti-tumor immunity; one was a tumor cell clone expressing a membrane-bound form of IL-12 p35 subunit (mbIL-12 p35 tumor clone), and the other was a tumor clone expressing heterodimeric IL-12 as a single chain (mb-scIL-12 tumor clone). The stimulatory effect of mb-scIL-12 on the proliferation of ConA-activated splenocytes was higher than that of mbIL-12 p35 in vitro. However, the stimulatory effect of mbIL-12 p35 was equivalent to that of recombinant soluble IL-12 (3 ng/ml). Interestingly, both tumor clones (mbIL-12 p35 and mb-scIL-12) showed similar tumorigenicity and induction of systemic anti-tumor immunity in vivo, suggesting that tumor cell expression of the membrane-bound p35 subunit is sufficient to induce anti-tumor immunity in our tumor vaccine model.
