Catalysis of lyase-isomerase PecE/PecF for several apophycobiliproteins.
- Author:
Jing-Ping ZHU
1
;
Ming ZHOU
;
Kai-Hong ZHAO
;
Zhi-Xiong ZENG
;
Yi-Kai ZHOU
Author Information
1. School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Apoproteins;
metabolism;
Bacterial Proteins;
Catalysis;
Cyanobacteria;
enzymology;
Light-Harvesting Protein Complexes;
Lyases;
metabolism;
Octoxynol;
pharmacology;
Proteins;
metabolism;
Substrate Specificity
- From:Chinese Journal of Biotechnology
2002;18(6):703-708
- CountryChina
- Language:Chinese
-
Abstract:
Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC alpha-subunit(alpha-PEC). In this work, the specificity of PecE/PecF on substrate apoproteins was reported. PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of alpha-PEC from two different subspecies of Mastigocladus laminosus, as well the site-directed mutated apoprotein of alpha-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin alpha-subunit(alpha-CPC) from Mastigocladus laminosus. The surfactant Triton X-100 had no effect for the reconstitution of alpha-PEC, while it could improve the reconstitution of PCB with apoprotein of alpha-CPC.
