Biocompatibility of SA/CS-CaCl2/PMCG Microcapsule in cell culture.
- Author:
Li-Yang ZHANG
1
;
Yi-Xin GUAN
;
Shan-Jing YAO
Author Information
1. Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
- Publication Type:Journal Article
- MeSH:
Alginates;
toxicity;
Biocompatible Materials;
toxicity;
Calcium Chloride;
toxicity;
Cellulose;
analogs & derivatives;
toxicity;
Escherichia coli;
drug effects;
growth & development;
Glucuronic Acid;
Hexuronic Acids;
Polymers;
toxicity;
Saccharomyces cerevisiae;
drug effects;
growth & development
- From:Chinese Journal of Biotechnology
2002;18(6):709-712
- CountryChina
- Language:Chinese
-
Abstract:
A novel multi-components microcapsule--SA/CS-CaCl2/PMCG system was introduced. The effects of PMCG and SA/CS-CaCl2/PMCG microcapsules on the growth of free E. coli and Saccharomyces cerevisiae were studied respectively. In addition, the growth of immobilized E. coli and Saccharomyces cerevisiae were also investigated. The results showed that: Just like other synthetic polycations, PMCG above certain concentration (0.5%) strongly inhibited the growth of free E. coli and Saccharomyces cerevisiae, but SA/CS-CaCl2/PMCG microcapsules almost had no effects on their growth and on the consumption of glucose concentration by Saccharomyces cerevisiae. What's more, immobilized E. coli and Saccharomyces cerevisiae grew almost as normally as free cultivation. As a whole, SA/CS-CaCl2/PMCG microcapsules had good biocompatiability and can be used as a new immobilization system.
