High density fed-batch culture of Escherichia coli DH5 alpha/pDH-B2m with DO feed-back control of nutrient feeding.
- Author:
Yi LI
1
;
Qin PU
;
Zhong-Liang ZHAO
;
Yu-Bo CHAI
;
Nan-Chun CHEN
;
Su-Min CHEN
Author Information
1. Department of Biochemistry and Molecular Biology, Forth Military Medical University, Xi'an 710032, China.
- Publication Type:Journal Article
- MeSH:
Bone Morphogenetic Protein 2;
Bone Morphogenetic Proteins;
biosynthesis;
genetics;
Culture Media;
Escherichia coli;
genetics;
Fermentation;
Glycerol;
pharmacology;
Recombinant Proteins;
biosynthesis;
Time Factors;
Transforming Growth Factor beta
- From:
Chinese Journal of Biotechnology
2002;18(6):718-723
- CountryChina
- Language:Chinese
-
Abstract:
Optimization of cultivation condition of recombinant E. coli DH5 alpha/pDH-B2m and the condition suitable for expression of recombinant mature peptide of human bone morphogenetic protein-2 was carried out in 500 mL shaking flasks and then transferred to NBS Bioflo IV, a 20 L DO feed-back fed-batch culture system, to obtain rhBMP-2. The results indicate that keeping dissolved oxygen at 40% and controlling nutrient feeding rate with DO feed back strategy can obtain theoretically 3.59 g recombinant mature peptide of hBMP-2 per liter of broth, the final cell density OD600 reaches 57(22.8 g dry cell weight/L), and the expression of rhBMP-2 amounts to 30% of the total protein in E. coli.
