Chemical constituents from roots of Caragana stenophylla and their anti-tumor activities.
10.19540/j.cnki.cjcmm.20170512.007
- Author:
Maiwulan SUBINUER
1
;
Lan PAN
2
;
Xin-Yue JIA
2
;
Tao ZHANG
3
;
Xiao-Guang JIA
2
;
Zhong-Mei ZOU
3
Author Information
1. School of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
2. Xinjiang Institute of Chinese Medicine and Ethnic Drug, Urumqi 830002, China.
3. Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Caragana stenophylla;
anti-tumor activity;
chemical constituents;
structure identification
- From:
China Journal of Chinese Materia Medica
2017;42(14):2697-2703
- CountryChina
- Language:Chinese
-
Abstract:
Fourteen compounds were isolated from the 80% ethanol extract of Caragana stenophylla root, by using a combination of various chromatographic approaches, including silica gel sephadex LH-20 column chromatography, and preparative HPLC. On the basis of their physical and chemical properties and spectroscopic data, their structures were elucidated as 2-(4-hydroxy-3-methoxy lphenyl)-3-methoxyl benzofuran-6-ol (1), mucodianin C (2), isopterofuran (3), formononetin (4), afromosin (5), calycosin (6), acacetin (7), 3-O-methylkaempferol (8), liquiritigenin (9), isoliquiritigenin (10), variabilin (11), resveratrol (12), zhebeiresinol (13), and 2, 3-dicarboxy-6, 7-dihydroxy-1-(3', 4'-dihydroxy)-phenyl-1, 2-dihydronaphthalen (14). Compound 1 is a new benzofuran derivative, named as mucodianin S; compounds 2, 3, 11, 13, 14 were isolated from the genus Caragana for the first time, and compounds 4-10 were firstly isolated from Caragana stenophylla. MTT assay was used to determine their cytotoxicity of the isolated compounds against human tumor cell lines, and 2 showed cytotoxicity against human hepato cellular cancer (HepG2) and human cervical (HeLa) lines, with IC₅₀ values of (16.18±0.95), (3.75±0.08) μmol•L ⁻¹, respectively.
