Construction of cell factories for high production of nerolidol in Saccharomyces cerevisiae.
10.19540/j.cnki.cjcmm.20170714.002
- Author:
Li-Li ZHANG
1
;
Xiao-Lin MA
1
;
Dong WANG
2
;
Peng YU
1
;
Lu-Qi HUANG
3
;
Xue-Li ZHANG
2
;
Zhu-Bo DAI
2
Author Information
1. College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
3. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Saccharomyces cerevisiae;
essential oils;
nerolidol;
synthetic biology
- From:
China Journal of Chinese Materia Medica
2017;42(15):2962-2968
- CountryChina
- Language:Chinese
-
Abstract:
Nerolidol is an important constituent of terpenoid essential oil and has excellent anti-tumor, anti-bacterial, and anti-oxidative properties. For realizing heterogenous production of nerolidol, our research firstly integrated the codon-optimized Actinidia sinensis nerolidol synthase gene (NES) into the terpenoid chassis strain FPP-001, and obtained NES-001 that could produce 2.71 mg•L⁻¹ nerolidol. Then, the N-terminal of the NES was fused with FPS by linker peptide GGGS. With this strategy, nerolidol production improved by 59.80-fold, reaching 162.07 mg•L⁻¹. Finally, by introduction of auxotrophic marker TRP1 in NES-002, the resulting strain NES-003 could produce 1 711.53 mg•L⁻¹ by high cell density fermentation method. This study provides the basis for the fermentative production of nerolidol and other sesquiterpenoids.
