High intracellular Mg²⁺ affects the activities of L-type calcium channel in guinea- pig ventricular myocytes.
- Author:
Mei-Mi ZHAO
1
;
Wen-Wen LIAN
;
Rui SUN
;
Hong-Mei WANG
;
Rui FENG
;
Hui-Yuan HU
;
Xue-Fei SUN
;
Li-Ying HAO
Author Information
1. Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang 110001, China. lyhao@mail.cmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium Channels, L-Type;
physiology;
Guinea Pigs;
Magnesium;
physiology;
Myocytes, Cardiac;
physiology;
Patch-Clamp Techniques
- From:
Acta Physiologica Sinica
2014;66(6):718-722
- CountryChina
- Language:Chinese
-
Abstract:
This study is aimed to investigate the effects of high intracellular Mg²⁺ on L-type calcium channel in guinea-pig ventricular myocytes. The cardiomyocytes were acutely isolated with enzyme digestion method. By adopting inside-out configuration of patch clamp technique, single channel currents of the L-type calcium channel were recorded under different intracellular Mg²⁺ concentrations ([Mg²⁺]i). In control group, which was treated with 0.9 mmol/L Mg²⁺, the relative activity of calcium channel was (176.5 ± 34.1)% (n = 7). When [Mg²⁺]i was increased from 0.9 to 8.1 mmol/L (high Mg²⁺ group), the relative activities of calcium channel decreased to (64.8 ± 18.1)% (n = 6, P < 0.05). Moreover, under 8.1 mmol/L Mg²⁺, the mean open time of calcium channel was shortened to about 25% of that under control condition (P < 0.05), but the mean close time of calcium channel was not altered. These results suggest that high intracellular Mg²⁺ may inhibit the activities of L-type calcium channel, which is mainly due to the shortening of the mean open time of single L-type calcium channel.
