Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: An In Vitro Study.
- Author:
Hyun Soo LEE
1
;
Soo Eun JUNG
;
Sue Kyung KIM
;
You Sun KIM
;
Seonghyang SOHN
;
You Chan KIM
Author Information
- Publication Type:Original Article
- Keywords: Collagen type I; Keloid fibroblast; Low-level light therapy
- MeSH: Blotting, Western; Collagen Type I; Collagen*; Dermis; Fibroblasts*; Humans*; In Vitro Techniques*; Keloid*; Low-Level Light Therapy*; Polymerase Chain Reaction; Reverse Transcription; Transforming Growth Factors
- From:Annals of Dermatology 2017;29(2):149-155
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Keloids are characterized by excessive collagen deposition in the dermis, in which transforming growth factor β (TGF-β)/Smad signaling plays an important role. Low-level light therapy (LLLT) is reported as effective in preventing keloids in clinical reports, recently. To date, studies investigating the effect of LLLT on keloid fibroblasts are extremely rare. OBJECTIVE: We investigated the effect of LLLT with blue (410 nm), red (630 nm), and infrared (830 nm) light on the collagen synthesis in keloid fibroblasts. METHODS: Keloid fibroblasts were isolated from keloid-revision surgery samples and irradiated using 410-, 630-, 830-nm light emitting diode twice, with a 24-hour interval at 10 J/cm². After irradiation, cells were incubated for 24 and 48 hours and real-time quantitative reverse transcription polymerase chain reaction was performed. Western blot analysis was also performed in 48 hours after last irradiation. The genes and proteins of collagen type I, TGF-β1, Smad3, and Smad7 were analyzed. RESULTS: We observed no statistically significant change in the viability of keloid fibroblasts after irradiation. Collagen type I was the only gene whose expression significantly decreased after irradiation at 410 nm when compared to the non-irradiated control. Western blot analysis showed that LLLT at 410 nm lowered the protein levels of collagen type I compared to the control. CONCLUSION: LLLT at 410 nm decreased the expression of collagen type I in keloid fibroblasts and might be effective in preventing keloid formation in their initial stage.
