Effect of Kv1.3 and KCa3.1 potassium ion channels on the proliferation and migration of monocytes/macrophages.
- Author:
Shuang-Xia ZHANG
1
;
Xian-Pei WANG
1
;
Chuan-Yu GAO
2
;
Chen-Hui JU
1
;
Li-Jie ZHU
1
;
Yi-Mei DU
3
Author Information
1. Department of Cardiology, Zhengzhou University People's Hospital, Zhengzhou 450003, China.
2. Department of Cardiology, Zhengzhou University People's Hospital, Zhengzhou 450003, China. chuanyu.gao@yahoo.com.
3. Research Center of Ion Channelopathy, Institute of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China.
- Publication Type:Journal Article
- MeSH:
Cell Movement;
Cell Proliferation;
Cnidarian Venoms;
pharmacology;
Enzyme-Linked Immunosorbent Assay;
Humans;
Kv1.3 Potassium Channel;
antagonists & inhibitors;
physiology;
Macrophages;
cytology;
Monocytes;
cytology;
Protein Structure, Tertiary;
Pyrazoles;
pharmacology;
Small-Conductance Calcium-Activated Potassium Channels;
antagonists & inhibitors;
physiology
- From:
Acta Physiologica Sinica
2015;67(5):505-512
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effects of blockade of Ca(2+) activated channel KCa3.1 and voltage-gated potassium channel Kv1.3 of the monocytes/macrophages on inflammatory monocyte chemotaxis. Chemotaxis assay was used to test the inflammatory Ly-6C(hi) monocyte chemotaxis caused by the monocytes/macrophages. The proliferation of monocytes/macrophages was detected by cell counting kit-8 (CCK8). Enzyme-linked immunosorbent assay (ELISA) was applied to detect the C-C motif ligand 7 (CCL7) in cultured media. The results showed that the recruitment of Ly-6C(hi) monocyte induced by monocytes/macrophages was suppressed by the potent Kv1.3 blocker Stichodactyla helianthus neurotoxin (ShK) or the specific KCa3.1 inhibitor TRAM-34. Meanwhile, the proliferation of monocytes/macrophages was significantly inhibited by ShK. The response of Ly-6C(hi) monocyte pretreated with ShK or TRAM-34 to CCL2 was declined. These results suggest that KCa3.1 and Kv1.3 may play an important role in monocytes/macrophages' proliferation and migration.
