Up-regulation of costimulatory molecules by sodium butyrate in acute leukemia cells and its molecular mechanism.
- Author:
Wei LI
1
;
Yu-li WANG
;
Li-hua KANG
;
Guan-jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: B7-1 Antigen; biosynthesis; B7-2 Antigen; biosynthesis; Butyrates; pharmacology; Cell Line, Tumor; Humans; Leukemia; metabolism; pathology; NF-kappa B; metabolism; Up-Regulation; drug effects
- From: Chinese Journal of Hematology 2005;26(5):265-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of sodium butyrate (SB) on the expression of costimulatory molecules in acute leukemia cells and its mechanism.
METHODSThe expression of CD86 and CD80 was examined on the surfaces of NB4, HL-60, Kasumi-1, U937 and Jurkat cells by flow cytometric analysis after treated by SB or not. Allogeneic mixed lymphocyte reaction was used to evaluate the immunomodulatory effects of cells treated by SB. Activated NF-kappaB was measured with an NF-kappaB assay kit.
RESULTSUp-regulation of CD86 and CD80 at various levels was observed on these leukemia cells treated by SB. The ratio of CD86 expressing cell in NB4 cells treated by 0.5 mmol/L SB was 36.8 times higher than that in control. Up-regulation of NF-kappaB was similar to that of CD86. Allogeneic lymphocyte proliferation was strongly stimulated by the SB treated cells.
CONCLUSIONSB can improve the expression of CD86 in acute leukemia cells. NF-kappaB was an important transcription factor involved in the up-regulation of CD86.
