MEKK2 regulates the production of interleukin 2.
- Author:
Xu-dong WEI
1
;
Rui-hua FAN
;
Xing-hu ZHU
;
Yu-fu LI
;
Yan-li ZHANG
;
Quan-li GAO
;
Yan-yan LIU
;
Ping WANG
;
Yong-ping SONG
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Interleukin-2; biosynthesis; genetics; Jurkat Cells; MAP Kinase Kinase 4; metabolism; MAP Kinase Kinase Kinase 2; metabolism; physiology
- From: Chinese Journal of Hematology 2005;26(5):296-298
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of MEKK2 on the production of IL-2 in Jurkat cells stimulated by PHA/anti-CD28 antibody.
METHODSThe MEKK2 and JNK kinase activities were measured in both dominant negative MEKK2 Jurkat (dnMEKK2 Jurkat) cells and parental Jurkat cells. The AP(1) and IL-2 promotor activities were measured by luciferase activity assay. The IL-2 mRNA and protein were detected by RT-PCR and Western blot.
RESULTSAfter stimulation by PHA/anti-CD28, JNK was activated in parental Jurkat cells but not in dnMEKK2 Jurkat cells. The luciferase report gene activities of AP1 and IL-2 promotors were increased by 4- and 5-folds in parental cells whereas only by 1 fold in dnMEKK2 Jurkat cells. The level of IL-2 mRNA and IL-2 protein were increased in parental Jurkat cells but not in dnMEKK2 Jurkat cells.
CONCLUSIONMEKK2 plays an important role on the production of IL-2 in Jurkat cell stimulated with PHA/anti-CD28 antibody. It is a potential drug target for the treatment of GVHD and autoimmune disease.