Detection of CCAAT/enhancer binding protein alpha gene mutations in acute myeloid leukemia.
- Author:
Jin-mei ZHAO
1
;
Hong-wei WANG
;
Zhi-fang XU
;
Lei ZHU
;
Bo BAI
;
Xiao-yan GE
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; CCAAT-Enhancer-Binding Protein-alpha; genetics; Child; Female; Humans; Leukemia, Myeloid, Acute; genetics; Male; Middle Aged; Mutation
- From: Chinese Journal of Hematology 2005;26(5):299-302
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between CCAAT/enhancer binding protein alpha (C/EBPalpha) gene mutations and the development of acute myeloid leukemia (AML).
METHODSThe whole coding region of C/EBPalpha gene were screened in 48 cases of AML and 11 normal subjects by PCR-single strand conformation polymorphism (PCR-SSCP) and sequencing.
RESULTSC/EBPalpha mutations were detected in 5 of 48 AML patients. Four duplications and 1 deletion were confirmed by DNA sequencing. All of those are newly identified mutations.
CONCLUSIONSDifferent mutation types of C/EBPalpha gene exist in a small number of patients with AML and might be related to the pathogenesis of some leukemias.
