Establishment of a rhesus haploidentical hematopoietic stem cell and mesenchymal stem cell transplantation model by nonmyeloablative conditioning.
- Author:
Li-hui LIU
1
;
Qi-yun SUN
;
Kai-xun HU
;
Ya-jing HUANG
;
Chuan-bo FAN
;
Zhao SUN
;
Bo YAO
;
Mei GUO
;
Chun-hua ZHAO
;
Hui-sheng AI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chimerism; Graft vs Host Disease; prevention & control; Haploidy; Hematopoietic Stem Cell Transplantation; Macaca mulatta; genetics; surgery; Mesenchymal Stem Cell Transplantation; Models, Animal; Transplantation Conditioning
- From: Chinese Journal of Hematology 2005;26(7):385-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish rhesus haploidentical hematopoietic stem cell transplantation model by nonmyeloablative conditioning, and examine the effects of mesenchymal stem cells (MSC) in haploidentical transplantation.
METHODSThe recipient haploidentical rhesus monkeys were conditioned with a nonmyeloablative regimen consisted of fludarabine, cyclophosphamide, 200 cGy total body irradiation, and rabbit anti-human thymocyte globulin. Cyclosporine A, mycophenolate mofetil and anti CD25 antibody were used for graft versus host disease (GVHD) prophylaxis. Rhesus monkeys in one group were given hematopoietic stem cell (HSC) only, while in the other group HSC combined with MSC. The differences in hematopoiesis recovery, chimerism level, and GVHD between the two groups were evaluated.
RESULTSStable chimerism could be achieved in recipient monkeys. Hematopoiesis recovery was mainly related with chimerism level. MSC seemed capable of facilitating HSC engraftment, as there were more mixed chimerism and less GVHD occurrence in the HSC combined with MSC recipient group.
CONCLUSIONA rhesus haploidentical hematopoietic stem cell transplantation model is successfully established by nonmyeloablative conditioning. MSC was of great benefit to haploidentical transplantation.
