The effect of HGF on graft-versus-host disease and graft-versus-leukemia after allogeneic bone marrow transplantation in acute lymphoblastic leukemia mice.
- Author:
Yun-jin XIA
1
;
Qing-ping GAO
;
Chu-cheng WAN
;
Fan-jun CHENG
;
Zhi-xiang LIU
;
Ren-ci GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Transplantation; Disease Models, Animal; Female; Graft vs Host Disease; prevention & control; Graft vs Leukemia Effect; drug effects; Hepatocyte Growth Factor; pharmacology; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Precursor Cell Lymphoblastic Leukemia-Lymphoma; immunology; surgery; Random Allocation; Transplantation, Homologous
- From: Chinese Journal of Hematology 2005;26(7):404-407
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hepatocyte growth factor (HGF) on graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) after allogeneic bone marrow transplantation (allo-BMT) and related mechanism in acute lymphoblastic leukemia (ALL) mice.
METHODSTwenty nude mice were randomly divided into control (group A) and test (group B) groups for monitoring relapse, and 20 BALB/c mice into control (group C) and test (group D) groups for GVHD. HGF as injected from day 0 to day 7 after BMT for groups B and D, while PBS for A and C. CD4(+) and CD8(+) T cell were evaluated by flow cytometry. The survival of mice after BMT was recorded. The level of tumor necrosis factor-alpha (TNF-alpha) was evaluated by ELISA.
RESULTSThe median past-BMT survival were 7.00 +/- 1.58, 9.00 +/- 1.58, 11.00 +/- 3.95 and 24.00 +/- 13.44 days for groups A, B, C, D, respectively, being prolonged in group D. HGF could decrease the quantity of CD4(+) T cells [group D (10.39 +/- 1.15)% vs group C (13.50 +/- 1.80)%, P < 0.01] and increase CD8(+) T cell [group D (12.25 +/- 2.85)% vs group C (6.12 +/- 1.99)%, P < 0.01], decrease the level of TNF-alpha in transplanted ALL mice [group D (112.10 +/- 18.99) pg/ml vs group C (143.90 +/- 25.35) pg/ml, P < 0.01] and reduce the degree of GVHD.
CONCLUSIONHGF could alleviate post-allo-BMT GVHD but retain GVL effect.
