Recipient lymphopenia state enhances the expansion and anti-leukemia effect of leukemia specific cytotoxic T lymphocytes.
- Author:
Jing-Wei LOU
1
;
Jian-Min YANG
;
Li CHEN
;
Xin-Yan JIA
;
Wei-Ping ZHANG
;
Jian-Min WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Female; Leukemia; immunology; Lymphopenia; immunology; Mice; Mice, Inbred C57BL; T-Lymphocytes, Cytotoxic; immunology
- From: Chinese Journal of Hematology 2005;26(8):465-468
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the role of recipient lymphopenia state in the expansion and function of leukemia specific cytotoxic T lymphocytes (CTLs).
METHODSC57BL/6 mice were induced to lymphopenia with 6 Gy total body irradiation. Spleen T cells or leukemia specific T cells from EGFP+ transgenic C57BL/6-EGFP mice were adoptively transferred by intravenous injection. The mice were challenged subcutaneously with 1 x 10(6) FBL3 leukemic cells at day 2 after irradiation. The peripheral WBC count, percentage of EGFP+ cells, subsets of T cells and tumor sizes were monitored.
RESULTBoth of the spleen T cell and leukemia specific CTL proliferated efficiently with the percentage of EGFP+ cells of 28. 81% and 42.24%, respectively, after infused into lymphopenic recipients. However, spleen T cells had no anti-leukemia effect regardless of its expansion. In contrast, leukemia specific CTLs showed a more rapid and extensive expansion under the condition of lymphopenia and a enhanced anti-leukemia immunity.
CONCLUSIONTransfusion of leukemia specific CTLs under lymphopenia state could be a feasible strategy to expand leukemia specific CTLs and generate favorable anti-leukemia effect in vivo.
