Multiplex fluorescence in situ hybridization in detecting complex chromosomal aberrations in myelodysplastic syndromes.
- Author:
Bing XIAO
1
;
Jian-yong LI
;
Jin-lan PAN
;
Li MA
;
Hai-rong QIU
;
Ya-fang WU
;
Yong-quan XUE
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Chromosome Aberrations; Female; Gene Rearrangement; Humans; In Situ Hybridization, Fluorescence; methods; Karyotyping; methods; Male; Middle Aged; Myelodysplastic Syndromes; genetics; Translocation, Genetic
- From: Chinese Journal of Hematology 2005;26(9):513-516
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the value of multiplex fluorescence in situ hybridization (M-FISH) technique in the detection of the complex chromosomal aberrations (CCAs) in myelodysplastic syndromes (MDS).
METHODSM-FISH was used in ten MDS patients with R-banding CCAs to refine the complex chromosomal rearrangements, the constitute of marker chromosomes, and to identify the cryptic translocations.
RESULTSThirty-seven kinds of structural rearrangements were detected by M-FISH including insertion, deletion, translocation and derivative chromosomes, among which 34 kinds were unbalanced rearrangements, and 3 were balanced rearrangements including t(6;22) (q21; q12), t(9; 19) (q13; p13) and t(3;5) (?; ?). Seven abnormalities in the present paper were first reported in the literature. In addition, chromosome 17 aberrations (7/10) and -5/5q - (7/10) were the two most frequent abnormalities.
CONCLUSIONSM-FISH could refine CCAs in MDS patients, find or correct the missed or misidentified abnormalities analysed by conventional cytogenetics.
