The effects of different transcripts of AML1 on the transactivation of M-CSF-R gene.
- Author:
Qing ZHANG
1
;
Min WANG
;
Hai-yan XING
;
Qing RAO
;
Jian-xiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; genetics; Cells, Cultured; Core Binding Factor Alpha 2 Subunit; genetics; Gene Expression Regulation, Leukemic; Genetic Vectors; Haplorhini; Hematopoietic Stem Cells; cytology; Kidney; cytology; Plasmids; genetics; Receptor, Macrophage Colony-Stimulating Factor; genetics; Transcriptional Activation; Transfection
- From: Chinese Journal of Hematology 2005;26(11):641-644
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of AML1A and AML1B, two splicing isoforms of AML1, on the transactivation of macrophage colony-stimulating factor receptor (M-CSF-R), and explore the mechanism of hematopoietic stem cell committed differentiation and leukemogenesis.
METHODSThe expressive plasmids of AML1A and AML1B were constructed, and co-transfected into CV-1 cells with a luciferase reporter plasmid containing M-CSF-R promoter. The transactivity of M-CSF-R promoter was assayed by luminometer.
RESULTSAML1B exhibited a distinct transactivity to M-CSF-R promoter with a sequence-specificity and dosage-dependent manner. AML1A showed no any transactivity but antagonized the effect of AML1B, causing marked reduction of M-CSF-R expression.
CONCLUSIONAn intact structure of AML1 is necessary for transactivation of M-CSF-R. AML1A may interfere with the transactivation of AML1B, and play a key role in the fine regulation of committed differentiation of hematopoietic cell.
