Exploration on the increased sensitivity to docetaxel and reversing mechanism of drug resistance of antisense survivin RNA in gastric cancer cell line SGC7901 cells.
- Author:
Rong MA
1
;
Xi-Hai CHEN
;
Qi-Fan ZHANG
;
Lei ZHU
;
Li-Ping TANG
;
Jing WANG
;
Hai-Yan CHENG
;
Li-Na LEI
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; biosynthesis; genetics; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Blotting, Western; Cell Line, Tumor; Cell Survival; drug effects; Drug Resistance, Neoplasm; drug effects; genetics; Electroporation; Humans; Inhibitor of Apoptosis Proteins; Inhibitory Concentration 50; Microtubule-Associated Proteins; biosynthesis; genetics; Neoplasm Proteins; biosynthesis; genetics; RNA, Antisense; genetics; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms; genetics; metabolism; pathology; Taxoids; pharmacology; Transfection; methods
- From: Chinese Journal of Oncology 2007;29(2):89-92
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of survivin antisense RNA on apoptosis and chemosensitivity to docetaxel in gastric cancer line SGC7901 cells, and its relation to mdr-1.
METHODSsurvivin antisense eukaryotic vector anti-pcDNA3-svv was transfected into SGC7901 cells by electorporation and positive clone was screened out. survivin protein and mdr-1 mRNA were determined by Western blot and RT-PCR. Apoptosis-inducing effect was examined by electron microscopy. Sensitivity to docetaxel was examined by MTT. Expression of mdr-1 and survivin mRNA were detected in the SGC7901 cells after drug-resisitance induction.
RESULTSThe expression of survivin protein of SGC7901 cells after transfection reduced significantly than that of non-transfected cells. MDR indexes of transfection group and non-transfection group were 0.196 +/- 0.013 and 3.126 +/- 0.019, respectively. The IC50 of transfection group to docetaxel was (16.7 +/- 1.98) microg/L and non-transfection group was (55.7 +/- 1. 89) microg/L, with a statistically significant difference. Expression of survivin mRNA in drug-resistant cells decreased along with the decreasing of mdr-1.
CONCLUSIONAntisense surivivin RNA can induce apoptosis in gastric cancer cells and increase sensitivity to docetaxel. The reversing mechanism of drug resistance is related with decreasing of mdr-1.
