Expression and biological activities of arresten in CHO cells.
- Author:
Miao-yun LONG
1
;
Qi-chang ZHENG
;
Zi-fang SONG
;
Qing-gang HU
;
Yong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Angiogenesis Inhibitors; biosynthesis; genetics; pharmacology; Animals; Blotting, Western; CHO Cells; Cell Line; Cell Movement; drug effects; Cells, Cultured; Collagen Type IV; biosynthesis; genetics; pharmacology; Cricetinae; Cricetulus; Endothelial Cells; cytology; drug effects; physiology; Humans; Neovascularization, Physiologic; drug effects; RNA, Messenger; biosynthesis; genetics; Recombinant Proteins; biosynthesis; genetics; pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Transfection
- From: Chinese Journal of Oncology 2007;29(4):249-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the eukaryotic expression of arresten in CHO cells and to investigate its basic biological activities.
METHODSCHO cells were divided into three groups: transfected pSecTag-arresten group, transfected pSecTag group and control group without transfection. PSecTag-arresten was transfected into CHO cells by Lipofectamine 2000 method. The arresten mRNA in CHO cells was assayed by RT-PCR. The protein expression of arresten gene was examined by Western-Blot. The cells expressing arresten were screened out by Zeocin. The effect of arresten on huvec cell migration and anchoring to three-dimensional vascular structures was measured.
RESULTSThe result of RT-PCR and Western-blot showed that arresten gene has been successfully transfected into CHO cells and expressed in those cells. Arrssten inhibited huvec cell migration and anchoring to three-dimensional vascular structures.
CONCLUSIONCHO cells expressing arresten have been obtained successfully. Arresten can inhibit huvec cell migration and anchoring to three-dimensional vascular structures, indicating that it might be one of its anti-angiogenetic approaches.
