MDR-reversing effect of short peptide binding specifically to multidrug-resistant gastric cancer cells.
- Author:
Peng WANG
1
;
Jie DING
;
Tao LIN
;
Shuang HAN
;
Shan-shan CAO
;
Fu-lin GE
;
Guang-qun AN
;
Rong LI
;
Ting LEI
;
Fei-hu BAI
;
Dai-ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; metabolism; pathology; Antibiotics, Antineoplastic; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Bacteriophages; genetics; Binding Sites; Cell Line, Tumor; Cell Membrane; metabolism; Cell Survival; drug effects; Doxorubicin; pharmacology; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Flow Cytometry; Fluorescent Antibody Technique; Humans; Peptide Library; Peptides, Cyclic; genetics; metabolism; Protein Binding; Stomach Neoplasms; genetics; metabolism; pathology; Vincristine; pharmacology
- From: Chinese Journal of Oncology 2007;29(4):258-261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the binding effect of the short peptide SY1 to the multidrug-resistant gastric cancer cell line SGC7901/VCR cells and its reversing effect on those cancer cells.
METHODSThe cultured cells were divided into two groups named SGC7901 and SGC7901/VCR. The SGC7901/VCR group was co-cultured with vincristine (VCR). SY1 was obtained from cyclic 7-mer peptide library by differential screening. Immunofluorescence technique was used to detect the capacity of SY1-containing positive phage specifically binding to SGC7901/VCR cells, compared with that of the negative phage and unrelated phage. MTT assay in vitro was performed to analyze the alteration of drug resistance of SGC7901/ VCR cells, using the positive phages and the chemically synthesized SY1 peptide. Flow cytometry assay was performed to detect the accumulation and retention of adriamycin (ADM) in the SGC7901/VCR cells.
RESULTSImmunofluorescence analysis showed that the SY1-containing positive phages could bind to the SGC7901/VCR cell surface but not to its parent cell line SGC7901 cells. The unrelated phage and negative phage did not bind to SGC7901/VCR cells. These results indicated that SY1 could specifically bind to SGC7901/VCR cells. MTT assay in vitro showed that the survival rate of SGC7901/VCR cells was reduced considerably by the positive phages and the chemically synthesized SY1 peptide (P <0. 05), indicating that SY1 enhanced the sensitivity of SGC7901/VCR cells to chemotherapeutic drug VCR. Flow-cytometric detection showed that SY1 enhanced the accumulation of ADM in the SGC7901/VCR cells, compared with that of the negative phages and the unrelated phages (P <0.05).
CONCLUSIONSY1 not only is able to bind to SGC7901/VCR cells specifically, but also can partly reverse the resistance of SGC7901/VCR cell line to chemotherapeutic drug VCR. Those findings might be important to open a new approach to reverse the gastric cancer MDR.